6J5M
OspA mutant, PSAM-VLGDV1-form3, grafted short chameleon sequence from alpha-B crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-11 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.142, 53.946, 65.937 |
| Unit cell angles | 90.00, 100.03, 90.00 |
Refinement procedure
| Resolution | 19.763 - 1.850 |
| R-factor | 0.1965 |
| Rwork | 0.194 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b2a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.757 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 0.665 |
| Number of reflections | 19236 | 935 |
| <I/σ(I)> | 24.6 | 2.84 |
| Completeness [%] | 97.6 | 96.1 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tri-HCl pH 8.5, 40% PEG 400 |
