6J5G
Complex structure of MAb 4.2-scFv with tick-borne encephalitis virus envelope protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.731, 100.693, 140.236 |
| Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
| Resolution | 46.000 - 3.291 |
| R-factor | 0.2084 |
| Rwork | 0.208 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1svb 5grj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.502 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.259 | 1.499 |
| Number of reflections | 28405 | 2816 |
| <I/σ(I)> | 36.2 | 6.2 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.979 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M sodium acetate pH 5, 5% PGA-LM, 8% PEG 20000 |
