6J5D
Complex structure of MAb 4.2-scFv with louping ill virus envelope protein Domain III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.598, 95.320, 100.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.261 - 1.800 |
| R-factor | 0.1707 |
| Rwork | 0.170 |
| R-free | 0.18890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1svb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.101 | 0.677 |
| Number of reflections | 42003 | 4127 |
| <I/σ(I)> | 16.4 | 0.5 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 13.1 | 12.2 |
| CC(1/2) | 0.999 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Na HEPES pH 7.5, 1.6M K/Na phosphate |
