6J5D

Complex structure of MAb 4.2-scFv with louping ill virus envelope protein Domain III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	I 2 2 2
Unit cell lengths	93.598, 95.320, 100.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.261 - 1.800
R-factor	0.1707
Rwork	0.170
R-free	0.18890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1svb
RMSD bond length	0.011
RMSD bond angle	1.028
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.101	0.677
Number of reflections	42003	4127
<I/σ(I)>	16.4	0.5
Completeness [%]	99.7	99.1
Redundancy	13.1	12.2
CC(1/2)	0.999	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Na HEPES pH 7.5, 1.6M K/Na phosphate