6J5B

Structural basis for the target DNA recognition and binding by the MYB domain of phosphate starvation response regulator 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-12
Detector	RAYONIX MX300HE
Wavelength(s)	0.978
Spacegroup name	P 1
Unit cell lengths	53.581, 53.581, 98.884
Unit cell angles	91.47, 91.47, 94.79

Refinement procedure

Resolution	39.440 - 2.700
R-factor	0.2195
Rwork	0.218
R-free	0.25080
RMSD bond length	0.008
RMSD bond angle	1.489
Data reduction software	xia2
Data scaling software	Aimless (0.7.3)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.520	47.520	2.830
High resolution limit [Å]	2.700	8.950	2.700
Rmerge	0.073	0.025	0.311
Rmeas	0.090	0.031	3.880
Rpim	0.052	0.018	2.276
Total number of observations	78501	1961	9784
Number of reflections	29308	775	3846
<I/σ(I)>	7.7	37.3	0.4
Completeness [%]	97.5	95.3	95.6
Redundancy	2.7	2.5	2.5
CC(1/2)	0.998	0.998	0.193

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	30% PEG 3350, 0.2M CaCl2, 0.1M MES pH 6.5