6J50
RNA polymerase II elongation complex bound with Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome (tilted conformation)
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TECNAI ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.7 |
Software | RELION,SerialEM |
Symmetry | C1 |
CTF correction | NONE |