6J4T
Crystal structure of arabidopsis ADAL complexed with IMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97849 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.630, 81.921, 87.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.820 |
R-factor | 0.18959 |
Rwork | 0.187 |
R-free | 0.22896 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j23 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.661 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.135 | |
Number of reflections | 32042 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 96.8 | |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Ammonium acetate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3350 |