6J4O
Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 83.140, 123.248, 195.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.310 - 2.300 |
| R-factor | 0.1874 |
| Rwork | 0.184 |
| R-free | 0.22390 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.047 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.134 | 1.000 |
| Rmeas | 0.146 | |
| Rpim | 0.058 | 0.459 |
| Number of reflections | 25255 | 1254 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 5.6 |
| CC(1/2) | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.2 | 293 | 1.6 M sodium/potassium phosphate, pH 8.2 |
