6J4M

Thermal treated soybean seed H-2 ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-04-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	I 2 3
Unit cell lengths	149.902, 149.902, 149.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.060 - 2.598
R-factor	0.195
Rwork	0.194
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a68
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.060	2.691
High resolution limit [Å]	2.598	2.598
Number of reflections	17380
<I/σ(I)>	4.5
Completeness [%]	100.0
Redundancy	11.5
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	Magnesium chloride, Tris, 1,6-hexylene glycol