6J4J

soybean seed H-2 ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	CCD
Collection date	2018-05-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97914
Spacegroup name	I 4
Unit cell lengths	130.827, 130.827, 171.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.700 - 2.101
R-factor	0.1615
Rwork	0.160
R-free	0.19130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a68
RMSD bond length	0.007
RMSD bond angle	0.746
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	2.177
High resolution limit [Å]	2.101	2.101
Number of reflections	83107
<I/σ(I)>	7.7
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.992	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	MPD, magnesium acetate tetrahydrate,sodium cacodylate trihydrate