6J4G
Crystal structure of the AtWRKY33 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 97.900, 97.900, 94.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.473 - 3.000 |
R-factor | 0.2079 |
Rwork | 0.204 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ayd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.168 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.474 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.175 | 0.800 |
Rmeas | 0.179 | |
Rpim | 0.035 | 0.470 |
Number of reflections | 5684 | 550 |
<I/σ(I)> | 13 | |
Completeness [%] | 98.8 | 100 |
Redundancy | 24.4 | 24.1 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8% v/v Ethylene glycol, 0.1M HEPES/Sodium hydroxide pH 7.5, 10% PEG 8000 |