6J4E

Crystal structure of the AtWRKY1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 65 2 2
Unit cell lengths	99.000, 99.000, 114.333
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.579 - 3.126
Rwork	0.203
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ayd
RMSD bond length	0.011
RMSD bond angle	1.115
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.580	3.238
High resolution limit [Å]	3.126	3.126
Rmerge	0.165	0.581
Rmeas	0.168	0.589
Number of reflections	6263	599
<I/σ(I)>	16.02
Completeness [%]	97.6	94.02
Redundancy	36.8	37.5
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Ammonium sulfate, 0.1M Sodium acetate trihydrate pH 4.6, 30% Polyethylene glycol monomethyl ether 2000