6J4B
Crystal structure of MarH, an epimerase for biosynthesis of Maremycins in Streptomyces, under 400 mM Zinc acetate
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97853, 1.2824, 1.2850 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 43.627, 43.627, 99.973 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.780 - 1.580 |
R-factor | 0.1475 |
Rwork | 0.146 |
R-free | 0.17720 |
Structure solution method | MAD |
RMSD bond length | 0.025 |
RMSD bond angle | 2.158 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CRANK |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 15724 | |
<I/σ(I)> | 49 | |
Completeness [%] | 99.9 | |
Redundancy | 19.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.4M Zinc acetate, 100 mM HEPES, pH 7.5, 4% PEG3350 |