6J4A

Human H chain ferritin with an extension peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-11-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	P 42 21 2
Unit cell lengths	219.800, 219.800, 148.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.309 - 3.990
R-factor	0.2867
Rwork	0.283
R-free	0.35110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fha
RMSD bond length	0.003
RMSD bond angle	0.536
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.309	4.133
High resolution limit [Å]	3.990	3.990
Number of reflections	30329
<I/σ(I)>	2.7
Completeness [%]	95.0
Redundancy	2
CC(1/2)	0.988	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	PEG 8000, Hepes, ethylene glycol