Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.229, 54.485, 66.489 |
Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
Resolution | 19.886 - 1.600 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.006 |
RMSD bond angle | 0.802 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.091 | 0.624 |
Number of reflections | 30305 | 1482 |
<I/σ(I)> | 25.9 | 1.97 |
Completeness [%] | 97.8 | 96.9 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 34% PEG 400, 0.1M Tris pH 8.8 |