Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.204, 53.173, 64.271 |
Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
Resolution | 27.002 - 1.900 |
R-factor | 0.2168 |
Rwork | 0.214 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.006 |
RMSD bond angle | 0.913 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.320 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.075 | 0.500 |
Number of reflections | 17284 | 833 |
<I/σ(I)> | 24 | 4.72 |
Completeness [%] | 98.3 | 96.9 |
Redundancy | 3.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 40% PEG 400, 0.1M Tris-HCl pH 9.0, protein 10 mg/mL |