6J46
LepI-SAH complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS PILATUS3 X CdTe 1M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.817, 62.333, 112.735 |
| Unit cell angles | 90.00, 112.45, 90.00 |
Refinement procedure
| Resolution | 53.202 - 2.621 |
| R-factor | 0.2105 |
| Rwork | 0.209 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j1o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.583 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.202 | 2.621 |
| High resolution limit [Å] | 2.621 | 2.621 |
| Rmerge | 0.093 | 0.827 |
| Number of reflections | 28684 | 14091 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 91.7 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.10M bis-tris pH 7.0, 35% PEG 400 |
