6J3H
Crystal structure of the glutathione S-transferase, CsGST83044, of Ceriporiopsis subvermispora in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 225.684, 48.397, 150.553 |
| Unit cell angles | 90.00, 129.27, 90.00 |
Refinement procedure
| Resolution | 48.763 - 2.190 |
| R-factor | 0.2028 |
| Rwork | 0.200 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f03 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.889 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 60962 | |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 93.7 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% (w/v) PEG 8000, 0.1M HEPES-HCl (pH7.5), 0.2M ammonium sulfate, 20% (v/v) 2-propanol |
