6J3E
Crystal structure of an apo form of the glutathione S-transferase, CsGST63524, of Ceriporiopsis subvermispora
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-15 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.236, 105.207, 91.703 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.629 - 2.455 |
R-factor | 0.1963 |
Rwork | 0.193 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f03 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.959 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 26458 | |
<I/σ(I)> | 30.13 | |
Completeness [%] | 98.6 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% (w/v) PEG 8000, 0.1M CHAPS-NaOH (pH 10.5), 0.2M NaCl |