6J36
crystal structure of Mycoplasma hyopneumoniae Enolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97892 |
Spacegroup name | I 4 |
Unit cell lengths | 193.154, 193.154, 63.943 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.289 - 2.301 |
R-factor | 0.1885 |
Rwork | 0.186 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mks |
RMSD bond length | 0.008 |
RMSD bond angle | 0.886 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.289 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.108 | 0.597 |
Number of reflections | 52577 | 39272 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.8 | |
Redundancy | 4.6 | |
CC(1/2) | 0.997 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 291 | 0.056 M sodium phosphate monobasic monohydrate, 1.344 M potassium phosphate dibasic, pH=8.4 |