6J2V

GABA aminotransferase from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	67.725, 70.495, 97.017
Unit cell angles	106.57, 96.48, 110.66

Refinement procedure

Resolution	33.610 - 1.900
R-factor	0.1484
Rwork	0.146
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.641
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.083	0.062	0.295
Rmeas	0.097	0.073	0.354
Rpim	0.051	0.039	0.194
Total number of observations	409512
Number of reflections	119202	5859	5801
<I/σ(I)>	14.6
Completeness [%]	97.2	95.6	95.4
Redundancy	3.4	3.5	3.1
CC(1/2)		0.990	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	25% PEG3350, 0.1M BIS-Tris (pH6.5), 0.2M Sodium chloride