6J2U

Crystal structure of Tyrosinase caddy protein(MelC1)with Tyrosinase (MelC2)from Streptomyces avermitilis in complex with Zinc ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	119.998, 71.828, 48.524
Unit cell angles	90.00, 113.72, 90.00

Refinement procedure

Resolution	60.120 - 1.300
R-factor	0.981485
Rwork	0.155
R-free	0.17210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z0d
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.120	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	91179	4459
<I/σ(I)>	29.3
Completeness [%]	98.4
Redundancy	3.5
CC(1/2)	0.993	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% polyethylene glycol 3350 and 0.2 M Lithium chloride