6J2U
Crystal structure of Tyrosinase caddy protein(MelC1)with Tyrosinase (MelC2)from Streptomyces avermitilis in complex with Zinc ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.998, 71.828, 48.524 |
Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
Resolution | 60.120 - 1.300 |
R-factor | 0.981485 |
Rwork | 0.155 |
R-free | 0.17210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z0d |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.120 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 91179 | 4459 |
<I/σ(I)> | 29.3 | |
Completeness [%] | 98.4 | |
Redundancy | 3.5 | |
CC(1/2) | 0.993 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% polyethylene glycol 3350 and 0.2 M Lithium chloride |