6J2E

Crystal structure of bat (Pteropus Alecto) MHC class I Ptal-N*01:01 in complex with Ebola virus-derived peptide EBOV-NP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-08-30
Detector	SDMS
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	100.740, 102.596, 177.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.284 - 2.100
R-factor	0.2084
Rwork	0.206
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.909
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	54243
<I/σ(I)>	2.365
Completeness [%]	100.0
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M succinic acid pH 7.0, 15%(w/v) polyethylene glycol 3,350