6J1Z
WWP2 semi-open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.031, 79.579, 58.870 |
| Unit cell angles | 90.00, 112.68, 90.00 |
Refinement procedure
| Resolution | 40.949 - 2.700 |
| R-factor | 0.211 |
| Rwork | 0.207 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nd7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.064 | 0.674 |
| Number of reflections | 12847 | 1210 |
| <I/σ(I)> | 22.9 | 2.6 |
| Completeness [%] | 98.3 | 97.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1M HEPES sodium pH 7.5, 10% v/v 2-Propanol, 20% w/v PEG 4000 |
