6J1K

Crystal structure of ribose-5-phosphate isomerase A from Ochrobactrum sp. CSL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	69.817, 169.262, 41.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.063 - 2.824
R-factor	0.2384
Rwork	0.236
R-free	0.28750
RMSD bond length	0.010
RMSD bond angle	1.434
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.063	2.925
High resolution limit [Å]	2.824	2.824
Number of reflections	11799
<I/σ(I)>	5.69
Completeness [%]	93.7
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	293	0.2M sodium citrate, 0.1M sodium chloride, PEG4000, 5mM DTT, pH 3.8