6J18
ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.161, 89.161, 62.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.608 - 2.000 |
| R-factor | 0.1684 |
| Rwork | 0.167 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nh0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.810 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.123 | 0.091 | 0.440 |
| Rmeas | 0.138 | 0.102 | 0.495 |
| Rpim | 0.062 | 0.046 | 0.226 |
| Total number of observations | 94374 | ||
| Number of reflections | 19280 | 1012 | 967 |
| <I/σ(I)> | 4.7 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 4.9 | 4.9 | 4.6 |
| CC(1/2) | 0.990 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M sodium dihydrogen phosphate monohydrate pH 4.5, 20% (w/v) PEG3350 |
