6J0Z

Crystal structure of AlpK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	APEX II CCD
Wavelength(s)	0.9793
Spacegroup name	P 61 2 2
Unit cell lengths	89.882, 89.882, 260.737
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.235 - 2.889
R-factor	0.2584
Rwork	0.256
R-free	0.30080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x4j
RMSD bond length	0.009
RMSD bond angle	1.491
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.240	2.992
High resolution limit [Å]	2.889	2.889
Rmerge	0.013	0.109
Number of reflections	14697	1204
<I/σ(I)>	25.89	5.58
Completeness [%]	95.0	83.98
Redundancy	2	2
CC(1/2)	1.000	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289.15	0.01M magnesium sulfate heptahydrate, 0.1M sodium Cacodylate, 0.8M ammonium sulfate