6J0Y
Crystal Structure of Yeast Rtt107 and Slx4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-31 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97855 |
Spacegroup name | P 1 |
Unit cell lengths | 63.450, 77.950, 78.380 |
Unit cell angles | 89.95, 79.15, 83.97 |
Refinement procedure
Resolution | 35.873 - 1.800 |
R-factor | 0.1605 |
Rwork | 0.159 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j0v |
Data scaling software | Aimless (0.3.11) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.150 | 55.150 | 1.840 |
High resolution limit [Å] | 1.790 | 8.010 | 1.790 |
Rmerge | 0.046 | 0.030 | 0.672 |
Rmeas | 0.053 | 0.036 | 0.777 |
Rpim | 0.027 | 0.019 | 0.389 |
Total number of observations | 532667 | 5442 | 39059 |
Number of reflections | 134886 | 1507 | 9805 |
<I/σ(I)> | 15 | 39.1 | 2.1 |
Completeness [%] | 97.6 | 97.5 | 96.1 |
Redundancy | 3.9 | 3.6 | 4 |
CC(1/2) | 0.999 | 0.997 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 277 | 10% PEG 6000, 0.1M HEPES pH 7.0, silver bullets additive G9 |