6J0Y

Crystal Structure of Yeast Rtt107 and Slx4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-31
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97855
Spacegroup name	P 1
Unit cell lengths	63.450, 77.950, 78.380
Unit cell angles	89.95, 79.15, 83.97

Refinement procedure

Resolution	35.873 - 1.800
R-factor	0.1605
Rwork	0.159
R-free	0.18500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j0v
Data scaling software	Aimless (0.3.11)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.150	55.150	1.840
High resolution limit [Å]	1.790	8.010	1.790
Rmerge	0.046	0.030	0.672
Rmeas	0.053	0.036	0.777
Rpim	0.027	0.019	0.389
Total number of observations	532667	5442	39059
Number of reflections	134886	1507	9805
<I/σ(I)>	15	39.1	2.1
Completeness [%]	97.6	97.5	96.1
Redundancy	3.9	3.6	4
CC(1/2)	0.999	0.997	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	10% PEG 6000, 0.1M HEPES pH 7.0, silver bullets additive G9