6J0W

Crystal Structure of Yeast Rtt107 and Nse6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97855
Spacegroup name	P 1 21 1
Unit cell lengths	71.777, 101.418, 86.559
Unit cell angles	90.00, 108.57, 90.00

Refinement procedure

Resolution	45.707 - 2.400
R-factor	0.2208
Rwork	0.218
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j0v
Data scaling software	Aimless (0.3.11)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	82.050	82.050	2.770
High resolution limit [Å]	2.400	4.790	2.400
Rmerge	0.086	0.041	0.484
Rmeas	0.100	0.048	0.564
Rpim	0.052	0.025	0.288
Total number of observations	173636	21045	61335
Number of reflections	46118	5819	16112
<I/σ(I)>	12.1	27.3	3.1
Completeness [%]	99.7	99.1	99.9
Redundancy	3.8	3.6	3.8
CC(1/2)	0.995	0.997	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	22% PEG 3350, 0.1M Bis-Tris pH 6.0, 0.2M ammonium acetate