6J0W
Crystal Structure of Yeast Rtt107 and Nse6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97855 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.777, 101.418, 86.559 |
Unit cell angles | 90.00, 108.57, 90.00 |
Refinement procedure
Resolution | 45.707 - 2.400 |
R-factor | 0.2208 |
Rwork | 0.218 |
R-free | 0.27080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j0v |
Data scaling software | Aimless (0.3.11) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 82.050 | 82.050 | 2.770 |
High resolution limit [Å] | 2.400 | 4.790 | 2.400 |
Rmerge | 0.086 | 0.041 | 0.484 |
Rmeas | 0.100 | 0.048 | 0.564 |
Rpim | 0.052 | 0.025 | 0.288 |
Total number of observations | 173636 | 21045 | 61335 |
Number of reflections | 46118 | 5819 | 16112 |
<I/σ(I)> | 12.1 | 27.3 | 3.1 |
Completeness [%] | 99.7 | 99.1 | 99.9 |
Redundancy | 3.8 | 3.6 | 3.8 |
CC(1/2) | 0.995 | 0.997 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 277 | 22% PEG 3350, 0.1M Bis-Tris pH 6.0, 0.2M ammonium acetate |