6J0T
The crystal structure of exoinulinase INU1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-17 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.00822 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 172.650, 172.650, 172.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.840 - 2.800 |
R-factor | 0.202 |
Rwork | 0.198 |
R-free | 0.27150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eqv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.840 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.098 | 0.256 |
Number of reflections | 32347 | 3177 |
<I/σ(I)> | 11.7 | 3 |
Completeness [%] | 99.9 | 99.94 |
Redundancy | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG4000, 0.1 M sodium cacodylate pH6.0, 0.2 M MgCl2 |