6J0Q
Crystal structure of P domain from GII.11 swine norovirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.237, 76.237, 241.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.18154 |
Rwork | 0.180 |
R-free | 0.21627 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pun |
RMSD bond length | 0.007 |
RMSD bond angle | 1.211 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 1.998 | 2.000 |
Rmerge | 0.140 | 0.489 |
Number of reflections | 49356 | 2396 |
<I/σ(I)> | 30 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.7 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris (pH 8.0), 16% (w/v) polyethylene glycol 3350, 6% (v/v) tacsimate (pH 8.0) |