6J0L
Crystal structure of intracellular B30.2 domain of BTN3A3 mutant in complex with sulfate ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-02 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.067, 73.470, 74.896 |
| Unit cell angles | 90.00, 111.39, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.17882 |
| Rwork | 0.176 |
| R-free | 0.23435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n7u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.058 | |
| Number of reflections | 28751 | 2900 |
| <I/σ(I)> | 31.37 | |
| Completeness [%] | 97.5 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | BIS-TRIS ,Ammonium sulfate |
