6J0J
Crystal structure of Proliferating Cell Nuclear Antigen from Leishmania donovani with an unexplained density near residues Pro229,Pr0267 and Ala285
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.460, 150.910, 170.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.020 - 3.100 |
| R-factor | 0.17554 |
| Rwork | 0.174 |
| R-free | 0.22327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h0t |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.053 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.020 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.062 | 0.600 |
| Number of reflections | 63855 | 4439 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, sodium malonate |
