6J0J

Crystal structure of Proliferating Cell Nuclear Antigen from Leishmania donovani with an unexplained density near residues Pro229,Pr0267 and Ala285

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9660
Spacegroup name	P 21 21 21
Unit cell lengths	135.460, 150.910, 170.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	113.020 - 3.100
R-factor	0.17554
Rwork	0.174
R-free	0.22327
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h0t
RMSD bond length	0.016
RMSD bond angle	2.053
Data reduction software	iMOSFLM
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.020	3.180
High resolution limit [Å]	3.100	3.100
Rmerge	0.062	0.600
Number of reflections	63855	4439
<I/σ(I)>	13.2
Completeness [%]	99.7
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, sodium malonate