6J09

Crystal structure of Haemophilus Influenzae BamA POTRA1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-10
Detector	MAR CCD 130 mm
Wavelength(s)	0.9793
Spacegroup name	P 62 2 2
Unit cell lengths	127.340, 127.340, 136.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.100 - 3.000
R-factor	0.19386
Rwork	0.191
R-free	0.25172
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6izs 2qdf
RMSD bond length	0.021
RMSD bond angle	2.449
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.100	3.110
High resolution limit [Å]	3.000	3.000
Rmerge		0.030
Number of reflections	13673	1347
<I/σ(I)>	14.2
Completeness [%]	99.9
Redundancy	25.3
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	potassium sodium tartrate