6J08

Crystal structure of human MAJIN and TERB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	C 2 2 2
Unit cell lengths	107.737, 128.767, 86.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.192 - 2.900
R-factor	0.2142
Rwork	0.212
R-free	0.26220
Structure solution method	SAD
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.000
High resolution limit [Å]	2.900	6.250	2.900
Rmerge	0.077	0.065	0.655
Rmeas	0.080	0.068	0.681
Rpim	0.022	0.020	0.184
Total number of observations	182312
Number of reflections	13769	1474	1354
<I/σ(I)>	7.3
Completeness [%]	99.9	99.6	100
Redundancy	13.2	12.1	13.5
CC(1/2)		0.995	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.8	291	100 mM HEPES pH 6.8, 0.2 M Potassium thiocyanate, 22% (w/v) Polyethylene glycol 3350, 25% (v/v) Glycerol
1	EVAPORATION	6.8	291	100 mM HEPES pH 6.8, 0.2 M Potassium thiocyanate, 22% (w/v) Polyethylene glycol 3350, 25% (v/v) Glycerol