6J02
Crystal structure of the SRCR domain of mouse SCARA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2017-06-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 39.274, 42.881, 45.469 |
Unit cell angles | 91.03, 110.81, 117.35 |
Refinement procedure
Resolution | 25.091 - 1.803 |
R-factor | 0.1825 |
Rwork | 0.180 |
R-free | 0.21010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1by2 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.903 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.316 |
Number of reflections | 21345 | 4117 |
<I/σ(I)> | 19.758 | |
Completeness [%] | 96.6 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | polyethylene glycol 300, polyethylene glycol 8000, Glycerol, calcium, Tris (pH 8.5) |