6J00
The RNA-dependent RNA polymerase domain of dengue 3 NS5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 161.520, 177.360, 58.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.519 - 2.140 |
R-factor | 0.2206 |
Rwork | 0.219 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j7u |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.700 | 2.200 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 46398 | 3359 |
<I/σ(I)> | 12.06 | |
Completeness [%] | 99.9 | |
Redundancy | 7.4 | |
CC(1/2) | 0.998 | 0.616 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 16% polyethylene glycol 10000 and 100 mM Tris-HCl (pH 8.0) |