6IZT

Crystal structure of Haemophilus Influenzae BamA POTRA3-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	62.680, 89.900, 110.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.100 - 2.030
R-factor	0.2111
Rwork	0.210
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j09
RMSD bond length	0.004
RMSD bond angle	0.870
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.100	2.100
High resolution limit [Å]	2.030	2.030
Number of reflections	41023	4041
<I/σ(I)>	15.1
Completeness [%]	99.9
Redundancy	22
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000