6IZC
Crystal structure of the chromosome-encoded beta-lactamase of Vibrio parahaemolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.467, 97.741, 65.008 |
Unit cell angles | 90.00, 92.36, 90.00 |
Refinement procedure
Resolution | 54.100 - 1.550 |
R-factor | 0.165 |
Rwork | 0.164 |
R-free | 0.18900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g68 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | autoPROC (1.0.4) |
Phasing software | MOLREP (11.4.05) |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.749 | 1.555 |
High resolution limit [Å] | 1.549 | 1.549 |
Rmeas | 0.078 | 0.567 |
Rpim | 0.029 | 0.228 |
Number of reflections | 71621 | 619 |
<I/σ(I)> | 17.1 | 3.1 |
Completeness [%] | 97.9 | 82.6 |
Redundancy | 6.9 | 6 |
CC(1/2) | 0.999 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5 mg/ml in 10 mM HEPES pH 7.5, 150 mM NaCl, and 1 mM DTT was mixed with 0.2 M Ammonium sulfate, 0.1 M sodium acetate pH 4.2, and 34% polyethylene glycol monomethyl ether 2000 (w/v) in 1:1 volume ratio. |