6IZ8

Crystal Structure of TagF from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 21 21 2
Unit cell lengths	93.719, 92.386, 151.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.570 - 2.700
R-factor	0.2041
Rwork	0.201
R-free	0.25380
RMSD bond length	0.008
RMSD bond angle	1.042
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	37098
<I/σ(I)>	36.9
Completeness [%]	99.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2.5M NaCl, 0.1 M imidazole (pH 8.0), 3.2 M NaCl, 0.1 M Bis-Tris propane (pH 7.0)