6IZ6
Crystal Structure Analysis of TRIC counter-ion channels in calcium release
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-20 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.7712 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 250.110, 250.110, 250.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.134 - 3.293 |
R-factor | 0.3276 |
Rwork | 0.326 |
R-free | 0.35420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iz5 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14rc2_3191) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.134 | 48.130 | 3.550 |
High resolution limit [Å] | 3.290 | 8.710 | 3.290 |
Rmerge | 0.213 | 0.088 | 6.966 |
Rmeas | 0.214 | 0.088 | 7.047 |
Rpim | 0.023 | 0.009 | 1.043 |
Number of reflections | 9924 | 679 | 1812 |
<I/σ(I)> | 15.7 | ||
Completeness [%] | 92.6 | 99.4 | 84.7 |
Redundancy | 82.8 | 92.7 | 44.2 |
CC(1/2) | 1.000 | 1.000 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 278 | PEG 350 MME, CaCl2, MES PH6.5 |