6IZ5

Crystal Structure Analysis of a Eukaryotic Membrane Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-21
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.7712
Spacegroup name	F 4 3 2
Unit cell lengths	250.009, 250.009, 250.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.114 - 3.701
R-factor	0.3117
Rwork	0.311
R-free	0.32740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iyu
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.114	48.110	4.050
High resolution limit [Å]	3.690	9.050	3.690
Rmerge	0.505	0.063	8.699
Rmeas	0.512	0.064	8.831
Rpim	0.084	0.011	1.492
Number of reflections	7646	606	1754
<I/σ(I)>	10.4
Completeness [%]	99.8	99.2	99.6
Redundancy	36.3	32.7	33.9
CC(1/2)	0.999	1.000	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	22% PEG 400, 50mM MgCl2, 100mM Glycine pH 9.0