6IZ1
Crystal Structure Analysis of a Eukaryotic Membrane Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-08 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9792 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 127.348, 127.348, 101.801 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.487 - 2.399 |
R-factor | 0.2187 |
Rwork | 0.217 |
R-free | 0.25670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iyu |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14rc2_3191) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.490 | 48.490 | 2.490 |
High resolution limit [Å] | 2.400 | 8.970 | 2.400 |
Rmerge | 0.140 | 0.035 | 5.454 |
Rmeas | 0.142 | 0.035 | 5.522 |
Rpim | 0.023 | 0.007 | 0.858 |
Total number of observations | 688445 | 13453 | 80857 |
Number of reflections | 17876 | 457 | 1996 |
<I/σ(I)> | 23.1 | 108.2 | 1.1 |
Completeness [%] | 90.9 | 99.4 | 99.9 |
Redundancy | 38.5 | 29.4 | 40.5 |
CC(1/2) | 1.000 | 1.000 | 0.482 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 278 | PEG MME 2000 |