6IZ0
Crystal Structure Analysis of a Eukaryotic Membrane Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-31 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9792 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 127.049, 127.049, 101.448 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.587 - 2.300 |
R-factor | 0.2198 |
Rwork | 0.219 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iyu |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14rc2_3191) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.590 | 41.590 | 2.270 |
High resolution limit [Å] | 2.200 | 9.080 | 2.200 |
Rmerge | 0.189 | 0.023 | 6.754 |
Rmeas | 0.191 | 0.024 | 6.848 |
Rpim | 0.030 | 0.004 | 1.113 |
Total number of observations | 1001482 | ||
Number of reflections | 24924 | 417 | 2029 |
<I/σ(I)> | 23.9 | ||
Completeness [%] | 99.6 | 96.6 | 96.1 |
Redundancy | 40.2 | 31.9 | 36.5 |
CC(1/2) | 1.000 | 1.000 | 0.359 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 278 | PEG MME 2000 |