6IYG

The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07 with Maltotetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978910
Spacegroup name	P 21 21 21
Unit cell lengths	46.280, 64.570, 168.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.436 - 1.500
R-factor	0.1683
Rwork	0.167
R-free	0.18580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iwk
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.436	1.518
High resolution limit [Å]	1.500	1.500
Number of reflections	80886	1124
<I/σ(I)>	2
Completeness [%]	98.6
Redundancy	6
CC(1/2)	0.843	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M ammonium sulfate, 0.02 M Tris-HCl, 0.02 M maltotetraose