6IYE

Crystal structure of peptidyl-tRNA hydrolase from Acinetobacter baumannii with 12% PEG 1500 at 1.55 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.96600
Spacegroup name	P 21 21 21
Unit cell lengths	33.795, 65.849, 75.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.900 - 1.550
R-factor	0.25381
Rwork	0.253
R-free	0.27691
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y9a
RMSD bond length	0.011
RMSD bond angle	1.637
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.900	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.110	0.560
Rmeas	0.138	0.660
Rpim	0.068	0.330
Number of reflections	25090
<I/σ(I)>	5.9	2.2
Completeness [%]	92.1
Redundancy	3.78
CC(1/2)	0.987	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	12% PEG 1500, 0.1M HEPES PH 7.5