6IYA
Structure of the DNA binding domain of antitoxin CopASO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 58.541, 60.949, 105.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.220 - 3.000 |
R-factor | 0.2493 |
Rwork | 0.247 |
R-free | 0.29810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gpe |
RMSD bond length | 0.002 |
RMSD bond angle | 0.583 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.088 | 0.782 |
Rmeas | 0.098 | 0.873 |
Rpim | 0.042 | 0.378 |
Number of reflections | 7302 | 729 |
<I/σ(I)> | 15.7 | 2.2 |
Completeness [%] | 90.4 | 92.2 |
Redundancy | 4.8 | 4.6 |
CC(1/2) | 0.974 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 279 | 0.2 M Sodium formate, 20% w/v Polyethylene glycol 3350 |