6IXR

Structure of Myo2-GTD in complex with Inp2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	63.508, 63.508, 225.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.446 - 2.854
R-factor	0.2121
Rwork	0.209
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f6h
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.000	55.000	3.010
High resolution limit [Å]	2.850	9.030	2.850
Rmerge	0.172	0.070	1.557
Rmeas	0.177	0.073	1.598
Rpim	0.041	0.018	0.355
Number of reflections	13042	494	1854
<I/σ(I)>	14.5
Completeness [%]	99.9	99.6	99.8
Redundancy	19	14.9	20.1
CC(1/2)	0.998	0.998	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	8% w/v PEG 4000, 0.2-0.3 M lithium/ammonium sulfate pH 7.5