6IXP
Structure of Myo2-GTD in complex with Mmr1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.387, 63.459, 169.077 |
| Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
| Resolution | 53.381 - 2.733 |
| R-factor | 0.2443 |
| Rwork | 0.242 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f6h |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.550 | 54.550 | 2.880 |
| High resolution limit [Å] | 2.730 | 8.640 | 2.730 |
| Rmerge | 0.091 | 0.035 | 1.136 |
| Rmeas | 0.099 | 0.039 | 1.224 |
| Rpim | 0.038 | 0.015 | 0.453 |
| Total number of observations | 192730 | 6248 | 31890 |
| Number of reflections | 28335 | 1013 | 4404 |
| <I/σ(I)> | 8.1 | 15.8 | 2.8 |
| Completeness [%] | 93.4 | 98.8 | 99.6 |
| Redundancy | 6.8 | 6.2 | 7.2 |
| CC(1/2) | 0.999 | 0.998 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% w/v PEG3350 and 0.2M sodium acetate |
