6IXO
Apo structure of Myo2-GTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.660, 72.164, 168.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.081 - 1.901 |
R-factor | 0.1751 |
Rwork | 0.174 |
R-free | 0.20630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f6h |
RMSD bond length | 0.014 |
RMSD bond angle | 1.259 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.106 | 0.053 | 1.905 |
Rmeas | 0.111 | 0.056 | 1.988 |
Rpim | 0.032 | 0.017 | 0.562 |
Number of reflections | 49545 | 2696 | 2455 |
<I/σ(I)> | 5.7 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 11.6 | 10.7 | 12.1 |
CC(1/2) | 0.998 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1 M potassium chloride, 1 M ammonium sulfate and 0.1 M HEPES pH 7.0 |