6IXG

Crystal structure of native apo SH3BP5 (P41)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 41
Unit cell lengths	79.958, 79.958, 94.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.979 - 3.801
R-factor	0.2439
Rwork	0.242
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ixe
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.870
High resolution limit [Å]	3.800	3.800
Number of reflections	5605	237
<I/σ(I)>	14.9
Completeness [%]	94.4
Redundancy	9.1
CC(1/2)	0.970	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4M Mg(NO3)2, 0.1M Tris-Cl pH 8.0, 22% PEG 8000